Collagen-binding nanoparticles for extracellular anti-inflammatory peptide delivery decrease platelet activation, promote endothelial migration, and suppress inflammation

Using Collagen Binding Poly(n-isopropylacrylamide) Nanoparticles to Prevent Intravascular Platelet Adhesion and Activation

Platelet Adhesion to Collagen and Collagen-Related Peptide Under Flow

Objective—Platelet stimulation by collagen and collagen-related peptides (CRPs) is associated with activation of protein tyrosine kinases. In the present study, we investigated the role of Src family tyrosine kinases in the initial adhesion events of human platelets to collagen and cross-linked CRP. Methods and Results—Under arterial flow conditions, a glycoprotein VI–specific substrate, cross-...

Peptide Encapsulated Nanoparticles for Brain Drug Delivery

BBB is acting as a highly selective membrane that separates the circulating blood from brain fluid [1]. This barrier is formed by endothelial cells which are connected to each other at “tight” junctions that create a complete seal between them. Consequently, only lipophilic molecules are able to cross the barrier. Hydrophilic substances, in contrast, are unable to cross the lipid walls unless a...

Extracellular-Regulated Kinase Activation and Cas/Crk Coupling Regulate Cell Migration and Suppress Apoptosis during Invasion of the Extracellular Matrix

Regulation of cell migration/invasion is important for embryonic development, immune function, and angiogenesis. However, migratory cells must also coordinately activate survival mechanisms to invade the extracellular matrix and colonize foreign sites in the body. Although invasive cells activate protective programs to survive under diverse and sometimes hostile conditions, the molecular signal...

Temporal and Pharmacological Characterization of Angiostatin Release and Generation by Human Platelets: Implications for Endothelial Cell Migration

Platelets play an important role in thrombosis and in neo-vascularisation as they release and produce factors that both promote and suppress angiogenesis. Amongst these factors is the angiogenesis inhibitor angiostatin, which is released during thrombus formation. The impact of anti-thrombotic agents and the kinetics of platelet angiostatin release are unknown. Hence, our objectives were to cha...